ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of diallyl thiosulfinate (DATS) on the proliferation of side population (SP) cells in multiple myeloma (MM) and its mechanism.</p><p><b>METHODS</b>RPMI-8226 and NCI-H929 cells were cultured, and the level of SP cells was detected by Hoechst33342 staining. The SP cells were cultured and treated with 10 µg/ml DATS, the CCK8 assay was carried out to examine the effect of DATS on the proliferation ability in SP cells, and plate colony-forming test was used to examine the colony-forming ability, the flow cytometry assay was carried out to examine the cell cycle, Western blot assay was used to examine the expression of cyclin D1, cyclin E, CDK2 and CDK4.</p><p><b>RESULTS</b>SP cells were detected in RPMI-8226 and NCI-H929 cells with a proportion of 3.17±0.98 and 2.65±0.61, respectively. DATS treatment could significantly inhibit the SP cells survival in a time-dependent manner, and also could significantly inhibit the colony forming. In addition, DATS treatment could significantly induce the G/S arrest and suppress the expression of cyclin D1, cyclin E, CDK2 and CDK4.</p><p><b>CONCLUSION</b>DATS can inhibit the proliferation and colony-forming of SP cells in multiple myeloma, and induce the G/S arrest that may be carried out via suppressing the expression of cyclin D1, cyclin E, CDK2 and CDK4.</p>
ABSTRACT
To analyze the background of Sini Decoction prescription, taking the clinical emergency treatment of shock syndrome by Sini Decoction as the main line, to explore the following three problems on rescuing shock syndrome faced by Zhang Zhong-jing: The first is how to use Aconiti Lateralis Radix Preparata (fuzi) to restore yang for saving from collapse, in the case that the blood capacity can not be quickly supplied; The second is how the active constituents in fuzi could be quickly absorbed, in the pathological state of the shock with severe gastrointestinal disturbance; The third is how to relieve the toxicity of fuzi, in the condition that the active components are not affected; The aim of the study is to reveal the scientificality and rationality in the theory of traditional Chinese medicine, which could provide the guidance for the application of classical prescription in modern clinic or the development of new Chinese materia medica.